Article
Rapid and accurate determination of zygosity in transgenic animals by real-time quantitative PCR.
Transgenic research - 1 Feb 2002
Tesson Laurent, Heslan Jean-Marie, Ménoret Séverine, Anegon Ignacio
Abstract excerpt
Successful identification of homozygous and heterozygous transgenic animals with currently available techniques demands tedious and time-consuming procedures with a high proportion of ambiguous results. Real-time PCR is a quantitative and extremely precise method with high throughput that could be applied to the analysis of large numbers of animals differing only by a factor of two in the amount of target...
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