Article
Characterization of glycosylated variants of beta-lactoglobulin expressed in Pichia pastoris.
Protein engineering - 1 Mar 2001
Kalidas C, Joshi L, Batt C
Abstract excerpt
Glycosylated variants of beta-lactoglobulin (BLG) were produced in the methylotrophic yeast Pichia pastoris to mimic the glycosylation pattern of glycodelin, a homologue of BLG found in humans. Glycodelin has three sites for glycosylation, corresponding to amino acids 63-65 (S1), 85-87 (S2) and 28-30 (S3) of BLG. These three sites were engineered into BLG to produce the variants S2, S12 and S123, which carried...
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