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Article

O-glycosylation of a recombinant carbohydrate-binding module mutant secreted by Pichia pastoris.

Journal of molecular microbiology and biotechnology - 1 Jan 2003

Boraston Alisdair B, Sandercock Linda E, Warren R Anthony J, Kilburn Douglas G

Abstract excerpt

Carbohydrate-binding modules (CBMs; previously called cellulose-binding domains) make excellent fusion partners for the immobilization or purification of polypeptides. However, their use in eukaryotic hosts has been limited by glycosylation, which interferes with the ability of the CBM to bind to cellulose. We have engineered the C-terminal carbohydrate-binding module from Cellulomonas fimi xylanase 10A such that...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 12673059
DOI: 10.1159/000068721

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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