Article
Isolation of peroxisome-defective CHO mutant cells using green fluorescent protein.
Cell biochemistry and biophysics - 1 Jan 2000
Ito M, Ito R, Miura S, Huang Y
Abstract excerpt
The authors constructed a recombinant green fluorescent protein (GFP) (PTS-GFP), which carries peroxisome targeting signal (PTS1 or PTS2) as an additional sequence, by polymerase chain reaction. The gene encoding for the recombinant GFP was constructed into an eukaryotic expression vector, and stable transformant of CHO cell expressing PTS-GFP was isolated, following the transfection of the plasmid encoding for...
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