Article
Newly identified Chinese hamster ovary cell mutants defective in peroxisome biogenesis represent two novel complementation groups in mammals.
European journal of cell biology - 1 Aug 1997
Tateishi K, Okumoto K, Shimozawa N, Tsukamoto T, Osumi T, Suzuki Y, Kondo N, Okano I, Fujiki Y
Abstract excerpt
We isolated peroxisome biogenesis mutants from Chinese hamster ovary (CHO) cells, using the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/ UV) method and wild-type CHO-K1 cells that had been stably transfected with cDNA encoding Pex2p (formerly peroxisome assembly factor-1, PAF-1). Three mutant cell clo...
Topics
- Acetyl-CoA C-Acyltransferase
- Acyl-CoA Oxidase
- Animals
- CHO Cells
- Catalase
- Cell Fusion
- Cricetinae
- Cytosol
- Digitonin
- Fibroblasts
- Genetic Complementation Test
- Humans
- Lauric Acids
- Mammals
- Membrane Proteins
- Microbodies
- Mutagenesis
- Mutation