Article
Isolation of Chinese hamster ovary cell pex mutants: two PEX7-defective mutants.
Biochemical and biophysical research communications - 26 Apr 2002
Yanago Eiko, Hiromasa Takahide, Matsumura Tsuyoshi, Kinoshita Naohiko, Fujiki Yukio
Abstract excerpt
We searched for novel Chinese hamster ovary (CHO) cell mutants defective in peroxisome biogenesis by an improved method using peroxisome targeting signal 2 (PTS2)-tagged enhanced green fluorescent protein (EGFP). From mutagenized TKaEG2 cells, the wild-type CHO-K1 stably expressing rat Pex2p and PTS2-EGFP, cell colonies resistant to the 9-(1(')-pyrene)nonanol/ultraviolet treatment were examined for intracellular...
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