Article
Isolation of a new peroxisome-deficient CHO cell mutant defective in peroxisome targeting signal-1 receptor.
Biochemical and biophysical research communications - 13 Jan 1997
Tsukamoto T, Bogaki A, Okumoto K, Tateishi K, Fujiki Y, Shimozawa N, Suzuki Y, Kondo N, Osumi T
Abstract excerpt
For the study of mechanism of peroxisome biogenesis, we attempted to isolate CHO cell mutants deficient in peroxisome biogenesis. We used as the parent strain a stable CHO transformant of rat PEX2 (formerly named peroxisome assembly factor-1) cDNA, to avoid unusually frequent isolation of Pex2 mu...
Topics
- Animals
- CHO Cells
- Catalase
- Cell Fusion
- Cricetinae
- DNA Primers
- Digitonin
- Genetic Complementation Test
- Humans
- Membrane Proteins
- Microbodies
- Mutation
- Peroxisomal Biogenesis Factor 2
- Peroxisome-Targeting Signal 1 Receptor
- Polymerase Chain Reaction