Article
A novel pex2 mutant: catalase-deficient but temperature-sensitive PTS1 and PTS2 import.
Biochemical and biophysical research communications - 24 May 2002
Akiyama Noriko, Ghaedi Kamran, Fujiki Yukio
Abstract excerpt
We searched for Chinese hamster ovary (CHO) cell mutants defective in peroxisome biogenesis by using peroxisome targeting sequence (PTS) of Pex3p (amino acid residues 1-40)-fused enhanced green fluorescent protein (EGFP). From mutagenized wild-type CHO-K1 cells stably expressing rat Pex2p and Pex3p(1-40)-EGFP, cell colonies resistant to the 9-(1(')-pyrene)nonanol/ultraviolet treatment were examined for...
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