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Article

Scale-up of recombinant cutinase recovery by whole broth extraction with PEG-phosphate aqueous two-phase.

Bioseparation - 1 Jan 2000

Costa M J, Cunha M T, Cabral J M, Aires-Barros M R

Abstract excerpt

A whole broth extraction using an aqueous two-phase system (ATPS) composed by 5% (w/w) PEG 3350 and 15% (w/w) phosphate was used for the scale-up extraction and isolation of a recombinant Fusarium solani pisi cutinase, an extracellular mutant enzyme expressed in Saccharomyces cerevisiae, containing a fusion peptide (WP)4. The experiments were carried out at three different scales (10 ml, 1 l and 30 l). Mixing...

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Identifiers

PMID: 11321521
DOI: 10.1023/a:1008162209596

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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