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Article

Normalized Screening of Protein Engineering Libraries by Split-GFP Crude Cell Extract Quantification.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018

Santos-Aberturas Javier, Dörr Mark, Bornscheuer Uwe T

Abstract excerpt

The different expression level and solubility showed by each protein variant represents an important challenge during screening campaigns: Usually, the total activity measurement constitutes the only criterion for identifying improved variants. This hampers the chances of finding interesting mutants, especially if the aim is to improve activity: On the one hand, interesting but poorly soluble variants will remain...

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Identifiers

PMID: 29086308
DOI: 10.1007/978-1-4939-7366-8_9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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