Article
Purification and in situ immobilization of lipase from of a mutant of Trichosporon laibacchii using aqueous two-phase systems.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences - 1 Apr 2010
Zhang Yuan-Yuan, Liu Jun-Hong
Abstract excerpt
The crude intracellular lipase (cell homogenate) from Trichosporon laibacchii was subjected to partial purification by aqueous two-phase system (ATPS) and then in situ immobilization by directly adding diatomites as carrier to the top PEG-rich phase of ATPS. A partition study of lipase in the ATP...
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