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Article

Expression and secretion of defined cutinase variants by Aspergillus awamori.

Applied and environmental microbiology - 1 Aug 1998

van Gemeren I A, Beijersbergen A, van den Hondel C A, Verrips C T

Abstract excerpt

Several cutinase variants derived by molecular modelling and site-directed mutagenesis of a cutinase gene from Fusarium solani pisi are poorly secreted by Saccharomyces cerevisiae. The majority of these variants are successfully produced by the filamentous fungus Aspergillus awamori. However, the...

Topics

Aspergillus
Blotting, Western
Carboxylic Ester Hydrolases
Electrophoresis, Polyacrylamide Gel
Fungal Proteins
Gene Expression Regulation, Fungal
Genetic Variation
HSP70 Heat-Shock Proteins
Mutagenesis, Site-Directed
Plasmids
Precipitin Tests

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Transformation, Genetic

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Identifiers

PMID: 9687432
DOI: 10.1128/AEM.64.8.2794-2799.1998
PMCID: PMC106774

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9687432-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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