Article
Interfacial binding of cutinase rather than its catalytic activity determines the steady state interfacial tension during oil drop lipid hydrolysis.
Chemistry and physics of lipids - 1 Feb 1999
Flipsen J A, van Schaick M A, Dijkman R, van der Hijden H T, Verheij H M, Egmond M R
Abstract excerpt
Hydrolysis of triglycerides by cutinase from Fusarium solani pisi causes in oil drop tensiometer experiments a decrease of the interfacial tension. A series of cutinase variants with amino acid substitutions at its molecular surface yielded different values of the steady state interfacial tension. This tension value poorly correlated with the specific activity as such nor with the total activity (defined as the...
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