Article
Combined use of fluorescent dyes and flow cytometry to quantify the physiological state of Pichia pastoris during the production of heterologous proteins in high-cell-density fed-batch cultures.
Applied and environmental microbiology - 1 Jul 2010
Hyka Petr, Züllig Thomas, Ruth Claudia, Looser Verena, Meier Christian, Klein Joachim, Melzoch Karel, Meyer Hans-Peter, Glieder Anton, Kovar Karin
Abstract excerpt
Matching both the construction of a recombinant strain and the process design with the characteristics of the target protein has the potential to significantly enhance bioprocess performance, robustness, and reproducibility. The factors affecting the physiological state of recombinant Pichia past...
Topics
- Biotechnology
- Cell Proliferation
- Colony Count, Microbial
- Culture Media
- Flow Cytometry
- Fluorescent Dyes
- Methanol
- Phenotype
- Pichia
- Recombinant Proteins