Article
A highly efficient ligand-regulated Cre recombinase mouse line shows that LoxP recombination is position dependent.
EMBO reports - 1 Apr 2001
Vooijs M, Jonkers J, Berns A
Abstract excerpt
Conditional gene inactivation using the Cre/loxP system is widely used, but the difficulty in properly regulating Cre expression remains one of the bottlenecks. One approach to regulate Cre activity utilizes a mutant estrogen hormone-binding domain (ERT) to keep Cre inactive unless the non-steroidal estrogen analog 4-hydroxytamoxifen (OHT) is present. Here we describe a mouse strain expressing Cre-ERT from the...
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