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Article

Molecular evolution by staggered extension process (StEP) in vitro recombination.

Nature biotechnology - 1 Mar 1998

Zhao H, Giver L, Shao Z, Affholter J A, Arnold F H

Abstract excerpt

We have developed a simple and efficient method for in vitro mutagenesis and recombination of polynucleotide sequences. The staggered extension process (StEP) consists of priming the template sequence(s) followed by repeated cycles of denaturation and extremely abbreviated annealing/polymerase-ca...

Topics

Bacillus subtilis
Evolution, Molecular
Gene Library
Mutagenesis
Mutation
Polymerase Chain Reaction
Protein Engineering
Recombinant Proteins
Recombination, Genetic
Sequence Analysis, DNA
Subtilisins
Templates, Genetic

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Identifiers

PMID: 9528005
DOI: 10.1038/nbt0398-258

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9528005-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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