Article
Escherichia coli cell cycle control genes affect chromosome superhelicity.
EMBO reports - 1 Dec 2000
Weitao T, Nordström K, Dasgupta S
Abstract excerpt
We have used ethidium bromide titration for direct measurement of the changes in the negative supercoiling of Escherichia coli chromosome caused by mutations inactivating the cell cycle functions mukB and seqA. The amounts of the intercalative agent required to relax the supercoiled chromosome in mukB and seqA mutants were lower and higher, respectively, than for the wild-type parent, confirming that these cell...
Topics
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Cell Cycle
- Cell Membrane
- Centrifugation, Density Gradient
- Chloroquine
- Chromosomal Proteins, Non-Histone
- Chromosomes
- DNA, Superhelical
- DNA-Binding Proteins
- Deoxyribonuclease EcoRI
- Dose-Response Relationship, Drug
- Electrophoresis, Agar Gel
- Escherichia coli
- Escherichia coli Proteins
- Ethidium
- Intercalating Agents
- Mutation