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Article

Topoisomerase mutants and physiological conditions control supercoiling and Z-DNA formation in vivo.

The Journal of biological chemistry - 5 Feb 1991

Jaworski A, Higgins N P, Wells R D, Zacharias W

Abstract excerpt

The influence of topoisomerase I and gyrase mutations in Escherichia coli on the supercoiled density of recombinant plasmids and the stability of left-handed Z-DNA was investigated. The formation of Z-DNA in vivo by dC-dG sequences of different lengths was used to determine the effective plasmid supercoil densities in the mutant strains. The presence of Z-DNA in the cells was detected by linking number and EcoRI...

Topics

DNA Topoisomerases, Type I
DNA Topoisomerases, Type II
DNA, Bacterial
DNA, Superhelical
Escherichia coli
Mutation
Nucleic Acid Conformation
Plasmids
Temperature

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 1846630

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1846630-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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