Partition functions of mini-F affect plasmid DNA topology in Escherichia coli.

Abstract excerpt

Efficient segregation of the low copy number plasmid mini-F is dependent on partition functions encoded by the plasmid sopABC genes. The sop region encodes proteins SopA and SopB and a cis-acting element, sopC, which may function as a centromere analog. The SopC segment contains 12 imperfect 43 bp repeats to which the SopB protein binds. We have found that mutations in the sop genes affect superhelicity of...

Topics

• Bacterial Proteins
• Base Sequence
• DNA, Recombinant
• DNA, Superhelical
• Escherichia coli
• Escherichia coli Proteins
• F Factor
• Gene Expression Regulation, Bacterial
• Molecular Sequence Data
• Mutation
• Nucleic Acid Conformation