Article
Long-term culture of human immunodeficiency virus type 1 resulting in loss of glycosylation sites.
Journal of medical virology - 1 Mar 2001
Lin H J, Siwak E B, Lauder I J, Hollinger F B
Abstract excerpt
Cultures of human immunodeficiency virus type 1 (HIV-1) provided a model for the study of mutations in the absence of host antibodies. Replicate cultures of biological and molecular clones of HIV-1 were passaged weekly for 30 or 34 weeks. Eight regions of HIV-1 genomic RNA were analyzed by means of single-strand conformation polymorphism analysis and nucleotide sequencing. Six mutations were detected in the...
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