Article
HLA-DQB1 sequencing-based typing updated.
Tissue antigens - 1 Apr 2007
van Dijk A, Melchers R, Tilanus M, Rozemuller E
Abstract excerpt
The use of direct sequencing as a typing strategy is well acknowledged. Direct sequencing identifies all sequence motifs including new polymorphisms in heterozygous sequences. The earlier protocols for human leukocyte antigen HLA-DQB1 Sequencing-Based Typing (SBT) frequently encounter preferential amplification of one of the alleles that can lead to unreliable sequences or even to allelic dropout. In our new...
Topics
- Alleles
- DNA Primers
- Exons
- Genetic Carrier Screening
- HLA-DQ Antigens
- HLA-DQ beta-Chains
- Histocompatibility Testing
- Humans
- Polymerase Chain Reaction
- Sequence Analysis, DNA