Article
Unambiguous high resolution genotyping of human leukocyte antigens.
Journal of immunological methods - 1 Jun 2017
Bradshaw Robert A, Dunn Paul P J
Abstract excerpt
We have developed a high resolution sequencing based typing method for genotyping Human Leukocyte Antigens (HLA) over a period of twenty years. The methods are based upon the separation of HLA alleles per locus at the initial amplification to simplify the analysis post-sequencing. The increasing discovery of polymorphism in HLA, manifested in new alleles, has necessitated the continuing development of this...
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