Article
Green fluorescent protein mutant as label in homogeneous assays for biomolecules.
Analytical biochemistry - 1 Feb 2001
Deo S K, Daunert S
Abstract excerpt
The green fluorescent protein (GFP) and its mutants have been extensively used to study various cellular processes and, more recently, as labels in binding assays. We have employed a mutant of GFP, an enhanced GFP (EGFP), in the development of homogeneous assays for biotin and cortisol. To demonstrate the feasibility of using EGFP as a label with different kinds of binders in the development of homogeneous...
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