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Article

Rational design of green fluorescent protein mutants as biosensor for bacterial endotoxin.

Protein engineering - 1 Jun 2002

Goh Yan Y, Frecer Vladimir, Ho Bow, Ding Jeak L

Abstract excerpt

Enhanced green fluorescent protein (EGFP) was selected as a signalling scaffold protein for design of a fluorescent biosensor for bacterial endotoxin [or lipopolysaccharide (LPS)]. Virtual mutagenesis was utilized to model EGFP variants containing binding sites for LPS and lipid A (LA), the bioactive component of LPS. Cationic amphipathic sequences of five alternating basic and hydrophobic residues were...

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Identifiers

PMID: 12082168
DOI: 10.1093/protein/15.6.493

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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