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Article

Dual detection of peptides in a fluorescence binding assay by employing genetically fused GFP and BFP mutants.

Analytical chemistry - 1 Oct 1999

Lewis J C, Daunert S

Abstract excerpt

A competitive fluorescence microplate assay based on a red-shifted green fluorescent protein (rsGFP) and a blue fluorescent protein (BFP) was developed for the detection of two model peptides in the same sample. The assay employed gene fusion to prepare the fluorescently labeled peptide conjugates. Specifically, plasmids were constructed in which the genes encoding for the two small peptides (less than 12 amino...

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PMID: 10517149
DOI: 10.1021/ac990404d

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

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