Article
Development of GFP-based biosensors possessing the binding properties of antibodies.
Proceedings of the National Academy of Sciences of the United States of America - 21 Jul 2009
Pavoor Tej V, Cho Yong Ku, Shusta Eric V
Abstract excerpt
Proteins that can bind specifically to targets that also have an intrinsic property allowing for easy detection could facilitate a multitude of applications. While the widely used green fluorescent protein (GFP) allows for easy detection, attempts to insert multiple binding loops into GFP to impart affinity for a specific target have been met with limited success because of the structural sensitivity of the GFP...
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