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Article

Protease-sensitive signalling by chemically engineered intramolecular fluorescent resonance energy transfer mutants of green fluorescent protein.

Biochimica et biophysica acta - 17 Sept 2004

Suzuki Miho, Ito Yoichiro, Savage Hannah Elizabeth, Husimi Yuzuru, Douglas Kenneth T

Abstract excerpt

The native cysteine residues of green fluorescent protein (GFP) at positions 48 and 70 were replaced by non-thiolic amino acids, and new cysteine sites were introduced at specific, surface positions. Based on molecular modeling of the GFP structure, the sites chosen for mutagenesis to Cys were glutamic acid at position 6 and isoleucine at position 229. These new, unique cysteine sites provided reactive thiol...

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PMID: 15358514
DOI: 10.1016/j.bbaexp.2004.06.009

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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