Article
High-throughput SNP genotyping by allele-specific PCR with universal energy-transfer-labeled primers.
Genome research - 1 Jan 2001
Myakishev M V, Khripin Y, Hu S, Hamer D H
Abstract excerpt
We have developed a new method for high-throughput genotyping of single nucleotide polymorphisms (SNPs). The technique involves PCR amplification of genomic DNA with two tailed allele-specific primers that introduce priming sites for universal energy-transfer-labeled primers. The output of red and green light is conveniently scored using a fluorescence plate reader. The new method, which was validated on nine...
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