Article
Retroviral vectors containing a variant dihydrofolate reductase gene for drug protection and in vivo selection of hematopoietic cells.
Stem cells (Dayton, Ohio) - 1 Jan 1998
Allay J A, Galipeau J, Blakley R L, Sorrentino B P
Abstract excerpt
Transfer of drug resistance genes to hematopoietic cells is being studied as a means to protect against the myelosuppression associated with cancer chemotherapy and as a strategy for the in vivo selection and amplification of genetically modified cells. The goal of this study was to test if retroviral-mediated gene transfer of a dihydrofolate reductase (DHFR) variant (L22Y) could be used for in vivo selection of...
Topics
- Adult
- Amino Acid Substitution
- Animals
- Antigens, CD
- Bone Marrow Cells
- CD24 Antigen
- Encephalomyocarditis virus
- Female
- Gene Transfer Techniques
- Genes, Reporter
- Genetic Therapy
- Genetic Variation
- Genetic Vectors
- Harvey murine sarcoma virus