Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Cloning and expression of canine O6-methylguanine-DNA methyltransferase in target cells, using gammaretroviral and lentiviral vectors.

Human gene therapy - 1 Apr 2004

Zaboikin Michail, Srinivasakumar Narasimhachar, Zaboikina Tatiana, Schuening Friedrich

Abstract excerpt

The human O(6)-methylguanine-DNA methyltransferase (MGMT) gene and its mutants have been used for in vivo selection of transduced hematopoietic stem cells with 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) alone or in combination with O(6)-benzylguanine (BG). To allow similar in vivo selection in dogs, without the risk of inducing an immune response, we have cloned the canine MGMT drug resistance gene. Comparison...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Division
Share to Dogs

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genetic Vectors

Share to Kidney

Share to Lentivirus

Share to Moloney murine leukemia virus

Share to O(6)-Methylguanine-DNA Methyltransferase

Share to Retroviridae

Source

Identifiers

PMID: 15053863
DOI: 10.1089/104303404322959533

Genes

MGMT

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Cloning and expression of canine O6-methylguanine-DNA methyltransferase in target cells, using gammaretroviral and lentiviral vectors.PMID 15053863Read ↗

Polyclonal chemoprotection against temozolomide in a large-animal model of drug resistance gene therapySemantic proximity Read ↗

Methylguanine methyltransferase–mediated in vivo selection and chemoprotection of allogeneic stem cells in a large-animal modelSemantic proximity Read ↗

Retroviral-mediated expression of the P140A, but not P140A/G156A, mutant form of O6-methylguanine DNA methyltransferase protects hematopoietic cells against O6-benzylguanine sensitization to chloroethylnitrosourea treatment.Semantic proximity Read ↗

Protection and in Vivo Selection of Hematopoietic Stem Cells Using Temozolomide, O6-Benzylguanine, and an Alkyltransferase-Expressing Retroviral VectorSemantic proximity Read ↗

Creation of human alkyltransferases resistant to O6-benzylguanine.Semantic proximity Read ↗

Interaction of mammalian O(6)-alkylguanine-DNA alkyltransferases with O(6)-benzylguanine.Semantic proximity Read ↗

The P140K mutant of human O(6)-methylguanine-DNA-methyltransferase (MGMT) confers resistance in vitro and in vivo to temozolomide in combination with the novel MGMT inactivator O(6)-(4-bromothenyl)guanine.Semantic proximity Read ↗

Lentiviral Transduction of P140K MGMT into Human CD34+ Hematopoietic Progenitors at Low Multiplicity of Infection Confers Significant Resistance to BG/BCNU and Allows Selection in VitroSemantic proximity Read ↗

In vivo selection of MGMT(P140K) lentivirus–transduced human NOD/SCID repopulating cells without pretransplant irradiation conditioningSemantic proximity Read ↗

Canine red blood cell thiopurine S-methyltransferase: companion animal pharmacogenetics.Semantic proximity Read ↗

Protection of hematopoietic cells against combined O6-benzylguanine and chloroethylnitrosourea treatment by mutant forms of O6-methylguanine DNA methyltransferase.Semantic proximity Read ↗

Drug selection of mutant methylguanine methyltransferase from different oncoretroviral backbones results in multilineage hematopoietic transgene expression in primary and secondary recipients.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.