Article
Cloning and expression of canine O6-methylguanine-DNA methyltransferase in target cells, using gammaretroviral and lentiviral vectors.
Human gene therapy - 1 Apr 2004
Zaboikin Michail, Srinivasakumar Narasimhachar, Zaboikina Tatiana, Schuening Friedrich
Abstract excerpt
The human O(6)-methylguanine-DNA methyltransferase (MGMT) gene and its mutants have been used for in vivo selection of transduced hematopoietic stem cells with 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) alone or in combination with O(6)-benzylguanine (BG). To allow similar in vivo selection in dogs, without the risk of inducing an immune response, we have cloned the canine MGMT drug resistance gene. Comparison...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Carmustine
- Cell Division
- Cells, Cultured
- Cloning, Molecular
- Dogs
- Genetic Vectors
- HIV-1
- Humans
- Kidney
- Lentivirus
- Molecular Sequence Data
- Moloney murine leukemia virus