Article
Retroviral transduction of a mutant dihydrofolate reductase-thymidylate synthase fusion gene into murine marrow cells confers resistance to both methotrexate and 5-fluorouracil.
Human gene therapy - 20 Mar 2003
Capiaux Gina M, Budak-Alpdogan Tulin, Takebe Naoko, Mayer-Kuckuk Philipp, Banerjee Debabrata, Maley Frank, Bertino Joseph R
Abstract excerpt
Gene transfer-based myeloprotection strategies against chemotherapy require the development of effective drug resistance genes or gene combinations. Our laboratory has previously generated drug-resistant mutants of dihydrofolate reductase (DHFR F/S) and thymidylate synthase (TS G52S) for myeloprotection against methotrexate (MTX) and 5-fluorouracil (5-FU), respectively. For the purpose of conferring dual...
Topics
- 3T3 Cells
- Animals
- Blotting, Western
- Bone Marrow Cells
- Cell Survival
- Cells, Cultured
- Drug Resistance
- Fluorouracil
- Gene Expression
- Genetic Vectors
- Male
- Methotrexate
- Mice