Article
The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel.
Cell - 1 Sept 2000
Minor D L, Lin Y F, Mobley B C, Avelar A, Jan Y N, Jan L Y, Berger J M
Abstract excerpt
Kv voltage-gated potassium channels share a cytoplasmic assembly domain, T1. Recent mutagenesis of two T1 C-terminal loop residues implicates T1 in channel gating. However, structural alterations of these mutants leave open the question concerning direct involvement of T1 in gating. We find in mammalian Kv1.2 that gating depends critically on residues at complementary T1 surfaces in an unusually polar interface....
Topics
- Amino Acid Substitution
- Animals
- Binding Sites
- Circular Dichroism
- Crystallography, X-Ray
- Electrophysiology
- Ion Channel Gating
- Kv1.2 Potassium Channel
- Models, Molecular
- Mutation
- Oocytes