Article
Genetic footprinting with mariner-based transposition in Pseudomonas aeruginosa.
Proceedings of the National Academy of Sciences of the United States of America - 29 Aug 2000
Wong S M, Mekalanos J J
Abstract excerpt
The complete DNA sequence of Pseudomonas aeruginosa provides an opportunity to apply functional genomics to a major human pathogen. A comparative genomics approach combined with genetic footprinting was used as a strategy to identify genes required for viability in P. aeruginosa. Use of a highly efficient in vivo mariner transposition system in P. aeruginosa facilitated the analysis of candidate genes of this...
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