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Article

Evidence for the prion hypothesis: induction of the yeast [PSI+] factor by in vitro- converted Sup35 protein.

Science (New York, N.Y.) - 28 Jul 2000

Sparrer H E, Santoso A, Szoka F C, Weissman J S

Abstract excerpt

Starting with purified, bacterially produced protein, we have created a [PSI(+)]-inducing agent based on an altered (prion) conformation of the yeast Sup35 protein. After converting Sup35p to its prion conformation in vitro, we introduced it into the cytoplasm of living yeast using a liposome transformation protocol. Introduction of substoichiometric quantities of converted Sup35p greatly increased the rate of...

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Identifiers

PMID: 10915616
DOI: 10.1126/science.289.5479.595

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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Research trail→

Click a neighbor to travelStep 1 · 12 closest

Evidence for the prion hypothesis: induction of the yeast [PSI+] factor by in vitro- converted Sup35 protein.PMID 10915616Read ↗

In vitro propagation of the prion-like state of yeast Sup35 protein.Semantic proximity Read ↗

The [PSI+] prion of yeast: a problem of inheritance.Semantic proximity Read ↗

Support for the prion hypothesis for inheritance of a phenotypic trait in yeast.Semantic proximity Read ↗

A prolonged chronological lifespan is an unexpected benefit of the [PSI+] prion in yeast.Semantic proximity Read ↗

Prion protein remodelling confers an immediate phenotypic switch.Semantic proximity Read ↗

C-terminal truncation of the Sup35 protein increases the frequency of de novo generation of a prion-based [PSI+] determinant in Saccharomyces cerevisiae.Semantic proximity Read ↗

Sup35p in Its Soluble and Prion States Is Packaged inside Extracellular Vesicles.Semantic proximity Read ↗

Expanding the prion model for the yeast [PSI+] element.Semantic proximity Read ↗

Strains of [PSI(+)] are distinguished by their efficiencies of prion-mediated conformational conversion.Semantic proximity Read ↗

Epigenetic regulation of translation reveals hidden genetic variation to produce complex traits.Semantic proximity Read ↗

Multiple Gln/Asn-rich prion domains confer susceptibility to induction of the yeast [PSI(+)] prion.Semantic proximity Read ↗

Propagation of the yeast prion-like [psi+] determinant is mediated by oligomerization of the SUP35-encoded polypeptide chain release factor.Semantic proximity Read ↗

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