Article
Splenocytes cultured in low concentrations of IL-2 generate NK cell specificities toward syngenic and allogenic targets.
Cellular immunology - 10 Jul 2000
Nissen M H, Jeppesen M, Claesson M H
Abstract excerpt
Splenocytes cultured in the presence of 30-60 units/ml IL-2 for 5 days develop natural killer activity toward syngeneic and allogeneic tumor cell targets. The IL-2 activated splenocytes, themselves, are partially resistant, whereas concanavalin A-activated T blast cells are completely resistant to killing. Surprisingly, major histocompatibility complex (MHC)-I-negative target cells are also resistant to natural...
Topics
- Animals
- Cell Count
- Cells, Cultured
- Cytotoxicity, Immunologic
- H-2 Antigens
- Interleukin-2
- Isoantigens
- Killer Cells, Natural
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C3H