Article
Generation of syngeneic anti-tumour double negative cells (CD4- CD8-), with cytotoxic activity against clones of different MHC class I expression.
Journal of immunogenetics - 1 Jan 2000
Caballero A, Garrido A, Algarra I, Perez M, Garrido F
Abstract excerpt
In vivo immunization and secondary culture techniques were used to generate cytotoxic T lymphocytes against a tumour cell clone obtained from a methylcholantrene-induced fibrosarcoma. Our CTLs differed from classical CTLs (Ly2+ and MHC restricted) in that our lymphocytes exhibited a non-MHC restr...
Topics
- Animals
- Antigens, Differentiation, T-Lymphocyte
- CD8 Antigens
- Clone Cells
- Cytotoxicity, Immunologic
- Flow Cytometry
- Histocompatibility Antigens Class I
- Lymphocyte Depletion
- Mice
- Mice, Inbred Strains
- Phenotype
- T-Lymphocytes
- Tumor Cells, Cultured