Article
Direct atomic force microscope imaging of EcoRI endonuclease site specifically bound to plasmid DNA molecules.
Proceedings of the National Academy of Sciences of the United States of America - 20 Aug 1996
Allison D P, Kerper P S, Doktycz M J, Spain J A, Modrich P, Larimer F W, Thundat T, Warmack R J
Abstract excerpt
Direct imaging with the atomic force microscope has been used to identify specific nucleotide sequences in plasmid DNA molecules. This was accomplished using EcoRI (Gln-111), a mutant of the restriction enzyme that has a 1000-fold greater binding affinity than the wild-type enzyme but with cleava...
Topics
- Binding Sites
- Chromosome Mapping
- Deoxyribonuclease EcoRI
- Microscopy, Atomic Force
- Mutation
- Plasmids
- Sequence Analysis