Article
Multiple interactions of the Asp(2.61(98)) side chain of the gonadotropin-releasing hormone receptor contribute differentially to ligand interaction.
Biochemistry - 18 Jul 2000
Flanagan C A, Rodic V, Konvicka K, Yuen T, Chi L, Rivier J E, Millar R P, Weinstein H, Sealfon S C
Abstract excerpt
Mutation of Asp(2.61(98)) at the extracellular boundary of transmembrane helix 2 of the gonadotropin-releasing hormone (GnRH) receptor decreased the affinity for GnRH. Using site-directed mutagenesis, ligand modification, and computational modeling, different side chain interactions of Asp(2.61(98)) that contribute to high-affinity binding were investigated. The conservative Asp(2. 61(98))Glu mutation markedly...
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