Article
Studies on the attenuation phenotype of polio vaccines: poliovirus RNA polymerase derived from Sabin type 1 sequence is temperature sensitive in the uridylylation of VPg.
Virology - 20 Jun 2000
Paul A V, Mugavero J, Yin J, Hobson S, Schultz S, van Boom J H, Wimmer E
Abstract excerpt
Determinants of temperature sensitivity and/or attenuation in Sabin type 1 poliovirus reside in the 5' NTR and coding sequences of the capsid proteins and viral RNA polymerase, 3D(pol). Previous studies have implicated at least two mutations in 3D(pol) of Sabin 1 vaccine strain [PV1(S)], includin...
Topics
- Amino Acid Sequence
- Amino Acid Substitution
- DNA-Directed RNA Polymerases
- HeLa Cells
- Humans
- Hydrogen Bonding
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Phenotype
- Poliovirus
- Poliovirus Vaccine, Oral
- Poly U
- Protein Binding
- Protein Biosynthesis