Article
Upf1p control of nonsense mRNA translation is regulated by Nmd2p and Upf3p.
Molecular and cellular biology - 1 Jul 2000
Maderazo A B, He F, Mangus D A, Jacobson A
Abstract excerpt
Upf1p, Nmd2p, and Upf3p regulate the degradation of yeast mRNAs that contain premature translation termination codons. These proteins also appear to regulate the fidelity of termination, allowing translational suppression in their absence. Here, we have devised a novel quantitative assay for translational suppression, based on a nonsense allele of the CAN1 gene (can1-100), and used it to determine the regulatory...
Topics
- Adaptor Proteins, Signal Transducing
- Amino Acid Transport Systems
- Codon, Nonsense
- Fungal Proteins
- Gene Expression Regulation, Fungal
- Membrane Transport Proteins
- Mutation
- Protein Biosynthesis
- RNA Caps
- RNA Helicases
- RNA, Messenger
- RNA-Binding Proteins
- Saccharomyces cerevisiae Proteins
- Suppression, Genetic