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Article

Purification and polymerization properties of two lethal yeast actin mutants.

Biochemical and biophysical research communications - 10 May 2000

Frieden C, Du J, Schriefer L, Buzan J

Abstract excerpt

The budding yeast Saccharomyces cerevisiae contains a single actin gene and the gene product, actin, is essential for growth. Two mutants of yeast actin that do not support yeast growth were prepared from yeast by coexpressing the mutant and a 6-histidine-tagged wild-type actin followed by separation of the wild-type and mutant actin using Ni-NTA chromatography as described elsewhere [Buzan, J., Du, J., Karpova,...

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PMID: 10799320
DOI: 10.1006/bbrc.2000.2650

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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