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Article

Histidine-tagged wild-type yeast actin: its properties and use in an approach for obtaining yeast actin mutants.

Proceedings of the National Academy of Sciences of the United States of America - 16 Mar 1999

Buzan J, Du J, Karpova T, Frieden C

Abstract excerpt

Wild-type and an N-terminal 6-histidine-tagged actin have each been expressed by using a yeast strain that contains the actin gene on a plasmid and not on the chromosome. Yeast strains have also been constructed that use two plasmids, one expressing the wild-type protein and the other the 6-histidine-tagged protein. Yeast cells can be grown with either plasmid alone or with both plasmids together and appear to be...

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Identifiers

PMID: 10077595
DOI: 10.1073/pnas.96.6.2823
PMCID: PMC15853

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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