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Article

A novel system for expressing toxic actin mutants in Dictyostelium and purification and characterization of a dominant lethal yeast actin mutant.

The Journal of biological chemistry - 21 Sept 2007

Noguchi Taro Q P, Kanzaki Noriko, Ueno Hironori, Hirose Keiko, Uyeda Taro Q P

Abstract excerpt

We have developed a novel system for expressing recombinant actin in Dictyostelium. In this system, the C terminus of actin is fused to thymosin beta via a glycine-based linker. The fusion protein is purified using a His tag attached to the thymosin beta moiety and then cleaved by chymotrypsin immediately after the native final residue of actin to yield intact actin. Wild-type actin prepared in this way was...

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PMID: 17656358
DOI: 10.1074/jbc.M703165200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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