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Article

Yeast actin with a mutation in the "hydrophobic plug" between subdomains 3 and 4 (L266D) displays a cold-sensitive polymerization defect.

The Journal of cell biology - 1 Dec 1993

Chen X, Cook R K, Rubenstein P A

Abstract excerpt

Holmes et al. (Holmes, K. C., D. Popp, W. Gebhard, and W. Kabsch. 1990. Nature [Lond.] 347: 44-49) hypothesized that between subdomains 3 and 4 of actin is a loop of 10 amino acids including a four residue hydrophobic plug that inserts into a hydrophobic pocket formed by two adjacent monomers on the opposing strand thereby stabilizing the F-actin helix. To test this hypothesis we created a mutant yeast actin...

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Identifiers

PMID: 8245125
DOI: 10.1083/jcb.123.5.1185
PMCID: PMC2119884

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