Article
Yeast actin with a mutation in the "hydrophobic plug" between subdomains 3 and 4 (L266D) displays a cold-sensitive polymerization defect.
The Journal of cell biology - 1 Dec 1993
Chen X, Cook R K, Rubenstein P A
Abstract excerpt
Holmes et al. (Holmes, K. C., D. Popp, W. Gebhard, and W. Kabsch. 1990. Nature [Lond.] 347: 44-49) hypothesized that between subdomains 3 and 4 of actin is a loop of 10 amino acids including a four residue hydrophobic plug that inserts into a hydrophobic pocket formed by two adjacent monomers on...
Topics
- Actins
- Adenosine Triphosphatases
- Adenosine Triphosphate
- Amino Acid Sequence
- Base Sequence
- Biopolymers
- Cold Temperature
- Deoxyribonucleases
- Enzyme Activation
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Myosins
- Protein Conformation
- Protein Denaturation
- Saccharomyces cerevisiae
- Viscosity