Cre-lox recombination in Escherichia coli cells. Mechanistic differences from the in vitro reaction.

doi:10.1016/0022-2836(92)90623-r

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Article

Cre-lox recombination in Escherichia coli cells. Mechanistic differences from the in vitro reaction.

Journal of molecular biology - 5 Aug 1992

Adams D E, Bliska J B, Cozzarelli N R

Abstract excerpt

The mechanism of the Cre recombinase of bacteriophage P1 in Escherichia coli cells was analyzed by topological methods in order to determine the important features of the in vivo reaction. Lambda infection was used to introduce the cre gene into cells containing plasmid substrates. The products of Cre resolution on substrates with directly repeated sites were predominantly free circles, even though decatenation...

Topics

Chromosome Inversion
Coliphages
DNA Nucleotidyltransferases
DNA, Superhelical
Escherichia coli
Gene Amplification
Genes, Viral
Genotype
Integrases
Kinetics
Models, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Nucleotidyltransferases

Plasmids

Recombination, Genetic

Restriction Mapping

Topoisomerase II Inhibitors

Transposases

Viral Proteins

Source

Open in PubMed

Identifiers

PMID: 1324323
DOI: 10.1016/0022-2836(92)90623-r

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1324323-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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