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Article

Mutants of Tn3 resolvase which do not require accessory binding sites for recombination activity.

The EMBO journal - 1 Mar 1999

Arnold P H, Blake D G, Grindley N D, Boocock M R, Stark W M

Abstract excerpt

Tn3 resolvase promotes site-specific recombination between two res sites, each of which has three resolvase dimer-binding sites. Catalysis of DNA-strand cleavage and rejoining occurs at binding site I, but binding sites II and III are required for recombination. We used an in vivo screen to detect resolvase mutants that were active on res sites with binding sites II and III deleted (that is, only site I...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 10064606
DOI: 10.1093/emboj/18.5.1407
PMCID: PMC1171230

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Source receipt: pubmed, retrieved 7 Sept 2026.

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