Mutations that increase the activity of the promoter of the Escherichia coli melibiose operon improve the binding of MelR, a transcription activator triggered by melibiose.

Abstract excerpt

MelR is an Escherichia coli transcription factor that activates expression of the melAB operon in response to the presence of melibiose in the environment. MelR stimulates transcription initiation at the melAB promoter by binding to four sites centered at positions -120.5, -100.5, -62.5, and -42.5 upstream of the transcript start point. In a previous study, we described a spontaneous mutant that exhibited...

Topics

• Base Sequence
• DNA
• DNA-Binding Proteins
• Escherichia coli
• Escherichia coli Proteins
• Melibiose
• Molecular Sequence Data
• Mutation
• Operon
• Promoter Regions, Genetic
• Regulatory Sequences, Nucleic Acid
• Trans-Activators