Article
Histidine-94 is the only important histidine residue in the melibiose permease of Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jan 1990
Pourcher T, Sarkar H K, Bassilana M, Kaback H R, Leblanc G
Abstract excerpt
Oligonucleotide-directed, site-specific mutagenesis has been utilized to modify the melB gene of Escherichia coli such that each of the seven His residues in the melibiose permease has been replaced with Arg. Replacement of His-213, His-442, or His-456 has no significant effect on permease activity, while permease with Arg in place of His-198, His-318, or His-357 retains more than 70% of wild-type activity. In...
Topics
- Arginine
- Base Sequence
- Escherichia coli
- Genes, Bacterial
- Histidine
- Membrane Transport Proteins
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Plasmids
- Protein Conformation