Article
The Bacillus subtilis mannose regulator, ManR, a DNA-binding protein regulated by HPr and its cognate PTS transporter ManP.
Molecular microbiology - 1 May 2013
Wenzel Marian, Altenbuchner Josef
Abstract excerpt
The transcriptional activator ManR of the Bacillus subtilis mannose utilization operon is composed of an N-terminal DNA-binding domain, two phosphotransferase system (PTS) regulation domains (PRDs), an EIIB(Bgl) - and an EIIA(Fru) -like domain. Site-specific mutagenesis of ManR revealed the role of conserved amino acids representing potential phosphorylation sites. This was investigated by β-galactosidase...
Topics
- Alleles
- Amino Acid Sequence
- Bacillus subtilis
- Bacterial Proteins
- Base Sequence
- DNA Footprinting
- DNA-Binding Proteins
- Deoxyribonuclease I
- Gene Expression Regulation, Bacterial
- Genes, Reporter
- Lac Operon
- Mannose
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phosphorylation
- Phosphotransferases
- Promoter Regions, Genetic
- Transcription Factors