Article
Conversion of temperature-sensitive to -resistant gene expression due to mutations in the promoter region of the melibiose operon in Escherichia coli.
The Journal of biological chemistry - 3 Jul 1998
Tamai E, Shimamoto T, Tsuda M, Mizushima T, Tsuchiya T
Abstract excerpt
The melibiose utilization system of Escherichia coli W3133, a derivative of K12, is nonfunctional between 37 and 42 degreesC. The reason for this temperature sensitivity was thought to be that the melibiose transporter (MelB) of W3133 cells was temperature-sensitive. A mutant W3133-2 has been iso...
Topics
- Base Sequence
- Carrier Proteins
- DNA, Bacterial
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Half-Life
- Melibiose
- Molecular Sequence Data
- Mutation
- Operon
- Temperature